期刊
ADVANCES IN MEDICAL SCIENCES
卷 58, 期 1, 页码 112-117出版社
MEDICAL UNIV BIALYSTOK
DOI: 10.2478/v10039-012-0069-8
关键词
Tegillarca granosa; K562/ADM tumor; multidrug resistance genes; apoptosis; mice
资金
- Science and Technology Development Projects of Shandong Province [2007GG20002011]
Purpose: To evaluate the effect of Haishengsu (HSS), a protein extract from Tegillarca granosa, on multidrug-resistance genes mdr1, BCR/ABL and sorcin in transplanted tumors. Material/Methods: Mice were inoculated subcutaneously with a drug resistant leukemia cell line K562/ADM. Tumorbearing animals were divided into control, adriamycin, HSS and combination therapy (adriamycin plus HSS) groups. Flow cytometry was used to detect apoptosis of tumor cells, and RT-PCR was used to evaluate the expression of mdr1, BCR/ABL and sorcin. Results: The apoptosis rate in the high (71.8%), medium (72.3%) and low doses HSS group (72.4%) was higher than in control (1.2%, p<0.01), adriamycin (34.4%, p<0.05) or combination therapy group (46.4%, p<0.05). The mean optical density of mdr1, BCR/ABL and sorcin in HSS groups was lower than in control, adriamycin and combination therapy group (p<0.01). The optical density of the three genes in high HSS group was lower than in medium and low HSS group (p<0.01). Conclusions: Haishengsu promotes apoptosis of drug-resistant K562/ADM tumors in mice in a dose-dependent manner. The pro-apoptotic effect of Haishengsu may be related to a reduced expression of multidrug-resistance genes mdr1, BCR/ABL and sorcin.
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