期刊
INTERNATIONAL DAIRY JOURNAL
卷 50, 期 -, 页码 50-57出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.idairyj.2015.06.004
关键词
-
资金
- [17.610.011-300]
- [12.610.008-300]
Methods for determination of milk cholesterol concentration were compared, with particular emphasis on an enzymatic method (ME). Bovine and human milk samples (n = 40) were pre-treated and analysed by three different protocols: (i) IDF Standard with gas chromatography (GC); (ii) direct saponification (DS) and GC; (iii) DS and ME with spectrophotometric determination. A simplified method (DS + ME) was developed with an acceptable coefficient of variation. The cholesterol concentration values assessed by DS + ME were higher compared with DS + GC. There were no statistically significant differences between the results obtained by the enzymatic and IDF method (P = 0.09). The mean cholesterol concentrations in bovine and human milk were 16.71 +/- 4.21 mg dL(-1) and 2.80 +/- 0.65 mg dL(-1), respectively. The new method developed was less time-consuming and more economical, and could be suitable for large-scale studies. High recovery (95%) obtained by ME indicates that the results are reliable and could be the basis for routine analysis. (C) 2015 Elsevier Ltd. All rights reserved.
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