期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 32, 页码 18104-18112出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b05328
关键词
graphene oxide; quantum dot; nanotoxicology; single cell; reactive oxygen species; metabolomics
资金
- Ministry of Education of China as an innovative team project [IRT 13024]
- National Natural Science Foundation of China [31170473, 21037002, 21307061, 21407085]
- Tianjin Natural Science Foundation [14JCQNJC08900]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [2013003112016]
- Postdoctoral Science Foundation of China [2014M550138]
The interactions between nanomaterials and cells are fundamental in biological responses to nanomaterials. However, the size-dependent synergistic effects of envelopment and internalization as well as the metabolic mechanisms of nanomaterials have remained unknown. The nanomaterials tested here were larger graphene oxide nanosheets (GONS) and small graphene, oxide quantum dots (GOQD). GONS intensively entrapped single-celled Chlorella vulgaris, and envelopment by GONS reduced the cell permeability. In contrast, GOQD-induced remarkable shrinkage of the plasma membrane and then enhanced cell permeability through strong internalization effects such as plasmolysis, uptake of nanomaterials, an oxidative stress increase, and inhibition of cell division and chlorophyll biosynthesis. Metabolomics analysis showed that amino acid metabolism was sensitive to nanomaterial exposure. Shrinkage of the plasma membrane is proposed to be linked to increases in the isoleucine levels. The inhibition of cell division and chlorophyll a biosynthesis was associated with decreases in aspartic acid and serine, the precursors of chlorophyll a. The increases in mitochondrial membrane potential loss and oxidative stress were correlated with an increase in linolenic acid. The above metabolites can be used as indicators of the corresponding biological responses. These results enhance our systemic understanding of the size-dependent biological effects of nanomaterials.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据