期刊
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
卷 100, 期 -, 页码 165-171出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2015.03.002
关键词
Benzonitrile; Benzamide; Rhodococcus sp MTB5; NHase; Amidase
资金
- University Grants Commission (UGC), New Delhi, India [37-168/2009(SR)]
- UGC-SAP programme [F.3-15/2009(SAP-DRS Phase-I)]
Rhodococcus sp. MTB5, a bacterial strain capable of utilizing benzonitrile as the sole source of carbon and nitrogen was isolated from a nitrile-contaminated agricultural soil sample by selective enrichment culture technique. This bacterial strain is capable of utilizing both aliphatic and aromatic nitriles. The production of ammonia was noticed during the growth of this strain in the medium supplemented with benzonitrile. The enzyme assay and GC studies revealed that benzonitrile degradation by Rhodococcus sp. MTB5 follows a NHase/amidase pathway involving benzamide and benzoic acid as metabolic intermediates. The resting cells grown on benzonitrile showed NHase and amidase activities of 0.155 and 0.261 units, respectively. The strain utilized 30 mM benzonitrile completely and could tolerate and grow in benzonitrile at concentration up to 60 mM. The cells grown on glucose and ammonium nitrate did not show the above enzymatic activities indicating the inducible nature of these enzymes. In addition to benzonitrile, the isolate was also able to utilize indole-3-acetonitrile and aliphatic nitriles, namely, propionitrile, butyronitrile and valeronitrile. Furthermore, the bacterium simultaneously utilizes different combinations of aromatic and aliphatic nitriles. These novel features make the bacterium a suitable candidate for the in-situ application of sites contaminated with both aliphatic and aromatic nitriles. (C) 2015 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据