期刊
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
卷 104, 期 -, 页码 112-117出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2015.05.018
关键词
Bacillus subtilis; Blood meal; Degree of hydrolysis; Degradation; Response surface methodology
资金
- National Natural Science Foundation of China [31472104]
- National Science and Technology Support Program [2013BAD10B03]
- China Agriculture Research System [CARS-36]
- Program for Innovative Research Team of Universities in Heilongjiang Province [2012TD003]
The effects of initial pH, inoculum size, rotation speed, degradation temperature and time on the degree of hydrolysis (DH) by Bacillus subtilis CICC 10265 which were generated by ultra-violet (UV) in blood meal degradation medium were evaluated in single-factor experiments. The degradation conditions were optimized through response surface methodology by implementing the Box-Behnken design based on single-factor experiments. The optimal conditions for hydrolysis were a degradation temperature of 33.8 degrees C, a degradation time of 69.36 h, a pH value of 7.0, an inoculum size of 7.2 x 10(7) CFU/mL and a rotation of speed 250 r/min. Under these conditions, the model predicted a DH of 62.18%, and it was experimentally determined to be 62.05% under the optimal conditions for degradation. Three main influencing factors are degradation temperature, degradation time and initial pH. (c) 2015 Elsevier Ltd. All rights reserved.
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