期刊
ADVANCED SYNTHESIS & CATALYSIS
卷 353, 期 13, 页码 2191-2215出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adsc.201100446
关键词
biocatalysis; directed evolution; enantioselectivity; enzyme catalysis; protein engineering; rational protein design
资金
- Alexander von Humboldt Foundation
- Deutsche Bundesstiftung Umwelt
- State Mecklenburg-Vorpommern
Modern tools for enzyme discovery and protein engineering substantially broadened the number of enzymes applicable for biocatalysis and helped to alter their properties such as substrate range, enantioselectivity, and stability under process conditions. In addition, these methods also enabled one to explore reactions for organic synthesis for which no suitable enzymes were available until recently. This review provides a summary of the different concepts and technologies, which are exemplified for various enzymes.
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