期刊
ADVANCED ROBOTICS
卷 28, 期 7, 页码 449-455出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/01691864.2013.876933
关键词
single cell diagnostics; cell mechanics; microcontact printing; power-law rheology; atomic force microscopy
类别
资金
- Japan Society for the Promotion of Science [23656055, 24106501]
- A-STEP Grant from Japan Science and Technology Agency [AS242Z00786P]
- Grants-in-Aid for Scientific Research [25286081, 24106501, 23656055] Funding Source: KAKEN
To investigate the mechanical properties of adherent cells under conditions where cell-to-cell interaction can be prevented and controlled, we used microcontact printing to pattern single cells without intercellular contacts and measured them with a custom-built atomic force microscopy (AFM) system. The motorized AFM system can measure individual cells over a large spatial range, enabling the measurement of cells in a microarray format. We tested single fibroblast cells and found that the power-law of their complex shear modulus is not significantly influenced by cell-to-cell contact. This method is effective for obtaining high-throughput measurements on single cells.
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