期刊
JOURNAL OF BIOMOLECULAR SCREENING
卷 15, 期 3, 页码 302-307出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1087057109357791
关键词
patch clamp; lipid bilayer; porins; GUVs; antibiotic translocation
资金
- EU [MRTN-CT-2005-019335]
A chip-based automated patch-clamp technique provides an attractive biophysical tool to quantify solute permeation through membrane channels. Proteo-giant unilamellar vesicles (proteo-GUVS) were used to form a stable lipid bilayer across a micrometer-sized hole. because of the small size and hence low capacitance of the bilayer, single-channel recordings were achieved with very low background noise. the latter allowed the characterization of the influx of 2 major classes of antibiotics cephalosporins and fluoroquinolones-through the major Escherichia coli porins OmpF and OmpC. Analyzing the ion current fluctuations in the presence of antibiotics revealed transport properties that allowed the authors to determine the mode of permeation. the chip-based setup allows rapid solution exchange and efficient quantification of antibiotic permeation through bacterial porins on a single-molecule level. (Journal of Biomolecular Screening 2010: 302-307)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据