期刊
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷 38, 期 3, 页码 325-331出版社
SCIENCE PRESS
DOI: 10.1016/S1872-2040(09)60030-X
关键词
Exosome; Non-small cell lung cancer; Sodium dodecylsulfonate-polyacrylate gel electrophoresis; High performance liquid chromatography-chip-tandem mass spectrometry
Urine provides an alternative to blood plasma as a potential source of disease biomarkers. Exosomes was separated by ultracentrifuge at 200000 g in normal persons and non-small cell lung cancer (NSCLC) patients' urine. For proteomic analysis of urinary exosome, 1D sodium dodecylsulfonate-polyacrylate gel electrophoresis(SDS-PAGE) was carries out and cut the gel 31 kDa - 20 kDa bands in normal group and disease group's. These gel blocks were subjected to in-gel trypsinization, and the extracted peptides were analyzed HPLC-CHIP-MS/MS. Approximately 24 unique proteins were identified in the UniProtKB/SWISS-PORT. The difference expression proteins were found in urinary exosome from NSCLC patients, including three fragment of the immunoglobulin kappa, two kinds of Ras related proteins, glutathione S-transferase A2, serum amyloid P-component precursor and phosphatidylethanolamine-binding protein 1.
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