期刊
ADVANCED FUNCTIONAL MATERIALS
卷 28, 期 39, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201801850
关键词
3D bioprinting; induced pluripotent stem cells; neural progenitor cells; spinal cord scaffolds; tissue engineering
类别
资金
- National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health [1DP2EB020537]
- CTSI KL2 Scholar Program of the National Institutes of Health [NIHCON000000033119-3002]
A bioengineered spinal cord is fabricated via extrusion-based multimaterial 3D bioprinting, in which clusters of induced pluripotent stem cell (iPSC)-derived spinal neuronal progenitor cells (sNPCs) and oligodendrocyte progenitor cells (OPCs) are placed in precise positions within 3D printed biocompatible scaffolds during assembly. The location of a cluster of cells, of a single type or multiple types, is controlled using a point-dispensing printing method with a 200 mu m center-to-center spacing within 150 mu m wide channels. The bioprinted sNPCs differentiate and extend axons throughout microscale scaffold channels, and the activity of these neuronal networks is confirmed by physiological spontaneous calcium flux studies. Successful bioprinting of OPCs in combination with sNPCs demonstrates a multicellular neural tissue engineering approach, where the ability to direct the patterning and combination of transplanted neuronal and glial cells can be beneficial in rebuilding functional axonal connections across areas of central nervous system (CNS) tissue damage. This platform can be used to prepare novel biomimetic, hydrogel-based scaffolds modeling complex CNS tissue architecture in vitro and harnessed to develop new clinical approaches to treat neurological diseases, including spinal cord injury.
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