期刊
ADVANCED FUNCTIONAL MATERIALS
卷 24, 期 18, 页码 2658-2666出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201302813
关键词
medical grade silicone; spider silk; capsular contracture; fibrosis; bioshields
类别
资金
- Interdisciplinary Center of Clinical Research (IZKF) a component of the Wurzburg University Hospital [A-106-N]
- [SFB 840 TPA8]
Medical grade silicones have been employed for decades in medical applications. The associated long-term complications, such as capsule formation and contraction have, however, not been fully addressed yet. The aim of this study is to elucidate if capsule formation and/or contraction can be mitigated by veiling the surface of the silicone during the critical phase after implantation. Medical grade silicone implants are homogeneously coated with a micrometer thin layer of recombinant spider silk proteins. Biocompatibility analysis in vitro and in vivo focuses on specific physiological reactions. Applying quantitative methods for the determination of marker-specific gene expression and protein concentration, it is detected that the silk coating inhibits fibroblast proliferation, collagen I synthesis, and differentiation of monocytes into CD68-positive histiocytes. It significantly reduces capsule thickness, post-operative inflammation, synthesis and re-modeling of extracellular matrix, and expression of contracture-mediating factors. Therefore, coatings made of recombinant spider silk proteins considerably reduce major post-operative complications associated with implantation of silicone-based alloprosthetics, such as capsular fibrosis and contraction, rendering spider silk coatings a bioshield for such implants.
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