Proteomics Approach to Investigate Impact of aFGF Antagonist on Signal Transduction Pathway

Fibroblast growth factor(FGF) signal transduction pathway is correlated with many diseases, such as cancer, skeletal dysplasias, olfactory syndromes and so on. P(VYMSPF), a small peptide, is an antagonist of aFGF. In order to investigate the impact of the peptide P on aFGF signal transduction pathway, the proteomes of NIH3T3 cell stimulated under different conditions(aFGF+heparin sodium and aFGF+heparin sodium+P) were extracted and analyzed by 2DE, five differentially expressed protein spots were selected and identified via ESI-MS/MS. The result indicates that the peptide P has a great influence on the function of aFGF such as metabolism, protein translation and cytoskeleton formation; the target signal pathway of the peptide P may be MAPK pathway and the peptide P might also affect the PLC gamma pathway. The article provides a new insight into the peptide P as a potential anticancer drug..