期刊
ADVANCED FUNCTIONAL MATERIALS
卷 24, 期 16, 页码 2396-2403出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201303400
关键词
micropatterning; UV lithography; mesenchymal stem cells; matrix elasticity; cell shape
类别
资金
- South Carolina Space Grant Consortium
- National Institute of Health [NIH P20 GM103641]
- National Science Foundation [EPS-0903795]
A wide variety of environmental factors including physical and biochemical signals are responsible for stem cell behavior and function. In particular, matrix elasticity and cell shape have been shown to determine stem cell function, yet little is known about the interplay between how these physical cues control cell differentiation. For the first time, by using ultraviolet (UV) lithography to pattern poly(ethylene) glycol (PEG) hydrogels, it is possible to manufacture microenvironments capable of parsing the effects of matrix elasticity, cell shape, and cell size in order to explore the relationship between matrix elasticity and cell shape in mesenchymal stem cell (MSC) lineage commitment. These data show that cells cultured on 1000 m(2) circles, squares, and rectangles are primarily adipogenic lineage regardless of matrix elasticity, while cells cultured on 2500 and 5000 m(2) shapes more heavily depend on shape and elasticity for lineage specification. It is further characterized how modifying the cell cytoskeleton through pharmacological inhibitors can modify cell behavior. By showing MSC lineage commitment relationships due to physical signals, this study highlights the importance of cell shape and matrix elasticity in further understanding stem cell behavior for future tissue engineering strategies.
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