期刊
ADVANCED FUNCTIONAL MATERIALS
卷 22, 期 11, 页码 2363-2375出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201200133
关键词
single-walled carbon nanotubes; protamine; mesenchymal stem cells; stem cell tracking; multimodal imaging
类别
资金
- National Basic Research Program (973 Program) of China [2012CB932600, 2011CB911002]
- National Natural Science Foundation of China [51132006, 51002100, 81171394]
- Natural Science Fund of Jiangsu Province [09KJB320016, SBK201122654]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Stem cells have shown great potential in regenerative medicine and attracted tremendous interests in recent years. Sensitive and reliable methods for stem cell labeling and in vivo tracking are thus urgently needed. Here, a novel approach to label human mesenchymal stem cells (hMSCs) with single-walled carbon nanotubes (SWNTs) for in vivo tracking by triple-modal imaging is presented. It is shown that polyethylene glycol (PEG) functionalized SWNTs conjugated with protamine (SWNT-PEG-PRO) exhibit extremely efficient cell entry into hMSCs, without affecting their proliferation and differentiation. The strong inherent resonance Raman scattering of SWNTs is used for in vitro and in vivo Raman imaging of SWNT-PEG-PRO-labeled hMSCs, enabling ultrasensitive in vivo detection of as few as 500 stem cells administrated into mice. On the other hand, the metallic catalyst nanoparticles attached on nanotubes can be utilized as the T2-contrast agent in magnetic resonance (MR) imaging of SWNT-labeled hMSCs. Moreover, in vivo photoacoustic imaging of hMSCs in mice is also demonstrated. The work reveals that SWNTs with appropriate surface functionalization have the potential to serve as multifunctional nanoprobes for stem cell labeling and multi-modal in vivo tracking.
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