期刊
INTEGRATIVE BIOLOGY
卷 7, 期 12, 页码 1534-1546出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ib00200a
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资金
- National Science Foundation [ECCS-0335765]
- National Institutes of Health [R01 NS059348, R01 HL082792]
- Department of Defense Breast Cancer Idea Award [BC102152]
- National Science Foundation CAREER award [CBET-1254846]
- Pilot Project Award by the Cornell Center on the Microenvironment & Metastasis through National Cancer Institute [U54CA143876]
- CDMRP [545346, BC102152] Funding Source: Federal RePORTER
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1254846] Funding Source: National Science Foundation
The ability of cells to migrate through tissues and interstitial spaces is an essential factor during development and tissue homeostasis, immune cell mobility, and in various human diseases. Deformation of the nucleus and its associated lamina during 3-D migration is gathering increasing interest in the context of cancer metastasis, with the underlying hypothesis that a softer nucleus, resulting from reduced levels of lamin A/C, may aid tumour spreading. However, current methods to study the migration of cells in confining three dimensional (3-D) environments are limited by their imprecise control over the confinement, physiological relevance, and/or compatibility with high resolution imaging techniques. We describe the design of a polydimethylsiloxane (PDMS) microfluidic device composed of channels with precisely-defined constrictions mimicking physiological environments that enable high resolution imaging of live and fixed cells. The device promotes easy cell loading and rapid, yet long-lasting (424 hours) chemotactic gradient formation without the need for continuous perfusion. Using this device, we obtained detailed, quantitative measurements of dynamic nuclear deformation as cells migrate through tight spaces, revealing distinct phases of nuclear translocation through the constriction, buckling of the nuclear lamina, and severe intranuclear strain. Furthermore, we found that lamin A/C-deficient cells exhibited increased and more plastic nuclear deformations compared to wild-type cells but only minimal changes in nuclear volume, implying that low lamin A/C levels facilitate migration through constrictions by increasing nuclear deformability rather than compressibility. The integration of our migration devices with high resolution time-lapse imaging provides a powerful new approach to study intracellular mechanics and dynamics in a variety of physiologically-relevant applications, ranging from cancer cell invasion to immune cell recruitment.
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