期刊
ADVANCED DRUG DELIVERY REVIEWS
卷 79-80, 期 -, 页码 3-18出版社
ELSEVIER
DOI: 10.1016/j.addr.2014.06.005
关键词
3D culture; Spheroids; Invasion assay; Xenograft; Basement membrane; Extracellular matrix; Angiogenesis; Dormancy assay
The basement membrane is an important extracellular matrix that is found in all epithelial and endothelial tissues. It maintains tissue integrity, serves as a barrier to cells and to molecules, separates different tissue types, transduces mechanical signals, and has many biological functions that help to maintain tissue specificity. A well-defined soluble basement membrane extract, termed BME/Matrigel, prepared from an epithelial tumor is similar in content to authentic basement membrane, and forms a hydrogel at 24-37 degrees C. It is used in vitro as a substrate for 3D cell culture, in suspension for spheroid culture, and for various assays, such as angiogenesis, invasion, and dormancy. In vivo, BME/Matrigel is used for angiogenesis assays and to promote xenograft and patient-derived biopsy take and growth. Studies have shown that both the stiffness of the BME/Matrigel and its components (i.e. chemical signals) are responsible for its activity with so many different cell types. BME/Matrigel has widespread use in assays and in models that improve our understanding of tumor biology and help define therapeutic approaches. (C) 2014 Elsevier B.V. All rights reserved.
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