期刊
ACTA VETERINARIA BRNO
卷 83, 期 -, 页码 S3-S8出版社
VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO
DOI: 10.2754/avb201483S10S3
关键词
Food safety; diarrhoeal enterotoxins; raw milk; pasteurized milk
资金
- Veterinary Aspects of Food Safety and Quality [MSM 6215712402]
- [IGA VFU 16/2013/FVHE]
The aim of this study was to compare Bacillus cereus growth rates and diarrhoeal enterotoxin production in raw and pasteurized goat, sheep, and cow milk in terms of storage conditions. Milk samples were inoculated with B. cereus (CCM 2010), which produces diarrhoeal enterotoxins. Enterotoxin production was tested by ELISA (Enzyme-Linked Immunosorbent Assay), and the count of B. cereus was determined by the plate method. With raw cow milk, B. cereus growth and enterotoxin production can be completely suppressed; in raw goat and sheep milk, enterotoxin was produced at 22 degrees C. In pasteurized cow, goat, and sheep milk, the B. cereus count increased under all storage conditions, with more rapid growth being observed at 15 degrees C (sheep milk) and 22 degrees C (cow and goat milk). Enterotoxin presence was detected at 15 degrees C and 22 degrees C, and with pasteurized cow milk also at 8 degrees C. Our model experiments have determined that B. cereus multiplication and subsequent enterotoxin production depend on storage temperature and milk type.
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