Diversity and abundance of mosquito species in relation to their larval habitats in Mizoram, North Eastern Himalayan region

The abundance, richness and diversity of anopheline and culicid mosquitoes associated with their habitats, season, and physico-chemical quality of water were surveyed along six districts of Mizoram, North Eastern Himalayan region. The productivity of permanent and temporary habitat types was quantified by carrying out weekly larval sampling using a standard dipping method for a period of three years. Diversity was estimated using the Shannon index (H'), Evenness index (Heve), similarity measures cluster analysis and MANOVA. In total, 5 genera and 20 species of mosquitoes were identified: Culex quinquefasciatus, Anopheles barbirostris and Anopheles vagus were the most abundant and widely-distributed species, representing 39.71%, 29.39% and 14.52% of total mosquito individuals sampled, respectively. Anopheles sp. diversity was lowest in Lunglei district (H' = 0.48) and highest in Aizawl (H' = 2.03), whereas Culex sp. diversity was lowest in Lawngtlai (H' = 0.38), and highest in Aizawl (H' = 2.99) and Kolasib (H' = 2.13). This represents the first update on the diversity and geographic distribution of the mosquitoes of Mizoram. Mosquito larvae were present in both temporary and permanent habitats suitable for breeding with monthly variations dependent on rainfall intensity, temperature, humidity and location. Early instars were more abundant significantly (P < 0.0001) than late instars among the habitat types in all study sites. The productivity of mosquito larvae was significantly (P < 0.0001) higher in ponds especially in permanent than semi-permanent and temporary. Weekly rainfall intensity led to an increase or decrease in anopheline and culicid larval abundance depending on the location. Mosquito diversity was highest in monsoon season (July-September) and lowest in January-March. A. barbirostris, A. vagus and C quinquefasciatus appear the most likely habitat generalist as it demonstrates both widespread distribution. Abundance and diversity of culicine and anopheline larvae were strongly associated (MANOVA) with pH, temperature, dissolved oxygen, alkalinity, phosphates and chlorides concentration of water. This information will be essential for designing and implementing mosquito larval control programs. (C) 2014 Elsevier B.V. All rights reserved.