期刊
ACTA TROPICA
卷 110, 期 1, 页码 46-51出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.actatropica.2009.01.003
关键词
Trichinella spiralis; Heat shock protein 70; Protective immunity
资金
- National Natural Science Foundation of China [30872201]
- Beijing Municipal project for Developing Advanced Human Resources for Education (BARED)
- Municipal Education Commission of Beijing [KZ200810025009]
- Project of Beijing Municipal Science & Technology Commission [Z080502036708012]
A cDNA encoding heat shock protein 70 of Trichinella spiralis (Ts-Hsp70) was identified by immunoscreening the adult T. spiralis cDNA library with rabbit antisera against T. spiralis adult extracts. The open reading frame of Ts-Hsp70 (DNA encoded a 623-amino acid peptide with a predicted molecular weight of 68.7 kDa, which shares a high degree of sequence conservation with HSP70s from other parasites. Recombinant Ts-Hsp70 was expressed in Escherichia coli and purified with nickel column chromatography. Western blot analysis showed that recombinant Ts-Hsp70 could be recognized not only by trichinellosis patient sera, but also by T. spiralis-infected sera from rabbits, swine, and mice. Mice vaccinated with recombinant Ts-Hsp70 formulated with Freund's adjuvant exhibited strong humoral immune responses indicated by high titer of IgG antibody and significant muscle larval reduction (37%) after being challenged with T. spiralis larvae. The present results indicate that Ts-Hsp70 is a possible candidate vaccine against T. spiralis infection. (c) 2009 Elsevier B.V. All rights reserved.
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