期刊
ACTA PHYSIOLOGICA
卷 210, 期 3, 页码 612-627出版社
WILEY
DOI: 10.1111/apha.12195
关键词
ageing muscle; central nuclei; master athlete; Pax7; satellite cell density
类别
资金
- Lundbeck Foundation
- Novo Nordisk Foundation
- Ministry of Culture Committee on Sports Research
- Danish Medical Research Council [10-094021]
- Danish Rheumatism Association [R92-A1592]
- MYOAGE [223576]
- European Commission
- Nordea Foundation (Healthy Ageing grant)
- NICHD
AimTo investigate the influence of lifelong endurance running on the satellite cell pool of type I and type II fibres in healthy human skeletal muscle. MethodsMuscle biopsies were collected from 15 healthy old trained men (O-Tr) who had been running 4316 (meanSD) kilometres a week for 28 +/- 9years. Twelve age-matched untrained men (O-Un) and a group of young trained and young untrained men were recruited for comparison. Frozen sections were immunohistochemically stained for Pax7, type I myosin and laminin, from which fibre area, the number of satellite cells, and the relationship between these variables were determined. ResultsIn O-Un and O-Tr, type II fibres were smaller and contained fewer satellite cells than type I fibres. However, when expressed relative to fibre area, the difference in satellite cell content between fibre types was eliminated in O-Tr, but not O-Un. A strong positive relationship between fibre size and satellite cell content was detected in trained individuals. In line with a history of myofibre repair, a greater number of fibres with centrally located myonuclei were detected in O-Tr. ConclusionLifelong endurance training (i) does not deplete the satellite cell pool and (ii) is associated with a similar density of satellite cells in type I and II fibres despite a failure to preserve the equal fibre type distribution of satellite cells observed in young individuals. Taken together, these data reveal a differential regulation of satellite cell content between fibre types, in young and old healthy men with dramatically different training histories.
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