期刊
ACTA PHYSIOLOGICA
卷 199, 期 1, 页码 71-81出版社
WILEY
DOI: 10.1111/j.1748-1716.2010.02074.x
关键词
gender; mTORC1 signalling; muscle contraction; protein metabolism
类别
资金
- National Institute for Arthritis and Musculoskeletal and Skin Diseases [R01 AR049877]
- Shared Instrumentation Grant Program [S10 RR16650]
- General Clinical Research Branch, National Center for Research Resources, National Institutes of Health [M01 RR00073]
- National Institute on Aging [P30 AG024832]
- National Institute on Disability and Rehabilitation Research, Department of Education [H133P040003]
- NIH [5T32HD007539]
Aim: Sex differences are evident in human skeletal muscle as the cross-sectional area of individual muscle fibres is greater in men than in women. We have recently shown that resistance exercise stimulates mammalian target of rapamycin (mTOR) signalling and muscle protein synthesis in humans during early post-exercise recovery. Therefore, the aim of this study was to determine if sex influences the muscle protein synthesis response during recovery from resistance exercise. Methods: Seventeen subjects, nine male and eight female, were studied in the fasted state before, during and for 2 h following a bout of high-intensity leg resistance exercise. Mixed muscle protein fractional synthetic rate was measured using stable isotope techniques and mTOR signalling was assessed by immunoblotting from repeated vastus lateralis muscle biopsy samples. Results: Post-exercise muscle protein synthesis increased by 52% in the men and by 47% in the women (P < 0.05) and was not different between groups (P > 0.05). Akt phosphorylation increased in both groups at 1 h post-exercise (P < 0.05) and returned to baseline during 2 h post-exercise with no differences between groups (P > 0.05). Phosphorylation of mTOR and its downstream effector S6K1 increased significantly and similarly between groups during post-exercise recovery (P < 0.05). eEF2 phosphorylation decreased at 1- and 2 h post-exercise (P < 0.05) to a similar extent in both groups. Conclusion: The contraction-induced increase in early post-exercise mTOR signalling and muscle protein synthesis is independent of sex and appears to not play a role in the sexual dimorphism of leg skeletal muscle in young men and women.
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