期刊
ACTA PHYSIOLOGIAE PLANTARUM
卷 35, 期 2, 页码 501-513出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11738-012-1092-x
关键词
ABA; Drought stress; Roots; Zea mays L.; Proteomics
资金
- National Natural Science Foundation of China [30800667, 31171470]
- Fok Ying-Tong Education Foundation, China [122032]
- Foundation of Henan Major Public Projects [091100910100]
- State Key Laboratory of Crop Biology [2011KF06]
We adopted a proteomics approach to identify and analyze the differential expression of maize root proteins associated with abscisic acid (ABA) regulation under combined drought and heat stress. Using mass spectrometry, we identified 22 major proteins that were significantly up-regulated under combined drought and heat stress. These 22 proteins were classified into 6 functional categories: disease/defense (8), metabolism (3), cell growth/division (3), signal transduction (2), transporters (2) and unclassified (4). Our previous reports showed that ABA regulates the expression of several small heat-shock proteins (sHSPs) in maize leaves subjected to the combination of drought and heat stress; however, no sHSPs were identified among the root proteins up-regulated in this study. RT-PCR and western blot analyses were used to identify six known sHSPs. The maize roots were pretreated with 100 mu M of ABA, and subsequently, the expression of the 22 up-regulated proteins and 6 sHSPs was examined. 11 proteins were up-regulated in an ABA-dependent manner, 13 proteins were up-regulated in an ABA-independent manner, and 4 proteins were up-regulated but inhibited by ABA. The up-regulated proteins are interesting candidates for further physiological and molecular investigations of combination stress tolerance in maize.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据