期刊
ACTA PHYSIOLOGIAE PLANTARUM
卷 33, 期 4, 页码 1467-1474出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11738-010-0683-7
关键词
Mass spectrometry; Pollen germination; Proteome; Two-dimensional gel electrophoresis; Zea mays
资金
- National Natural Science Foundation of China [30971705]
- Program for Science and Technology Innovation Talents in Universities of Henan Province [2008HASTIT005]
- Innovation Scientists and Technicians Troop Construction Projects of Henan Province [9410051003]
The identification of proteins involved in pollen germination and tube growth is important for fundamental studies of fertility and reproduction in flowering plants. We used 2-DE and MALDI-TOF-MS to identify differentially expressed proteins in mature (P0) and 1-h germinated (P1) maize pollen. Among about 470 proteins separated in 2D gels, the abundances of 26 protein spots changed (up- or down-regulation) between P0 and P1. The 13 up-regulated protein spots were mainly involved in tube wall modification, actin cytoskeleton organization, energy metabolism, signaling, protein folding and degradation. In particular, pectin methylesterase, inorganic pyrophosphatase, glucose-1-phosphate uridylyltransferase and rab GDP dissociation inhibitor alpha are highly up-regulated, suggesting their potential role in pollen tube growth. The down-regulated 13 protein spots mainly include defense-related proteins, pollen allergens and some metabolic enzymes. This study would contribute to the understanding of the changes in protein expression associated with pollen tube development.
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