期刊
ACTA PHYSIOLOGIAE PLANTARUM
卷 32, 期 3, 页码 591-596出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11738-009-0437-6
关键词
Desiccation; Cryopreservation; Liquid nitrogen; Water content; Seed storage
资金
- State Committee for Scientific Research, Poland [5 P06H 057 18]
To evaluate the feasibility of long-term cryopreservation of Polish provenances of silver birch (Betula pendula), the sensitivity of conditionally dormant seeds to extreme desiccation and/or the ultra-low temperature of liquid nitrogen (LN; -196A degrees C) was evaluated. The critical water content (WC) of desiccated seeds and the high-moisture freezing limit of seeds desiccated or moistened to various WCs and frozen for 24 h or for 2 years in LN was also determined. Germination tests revealed no critical WC for seeds [to 0.02 g H(2)O g(-1) dry mass (g g(-1))]. Seeds tolerated freezing in LN within specific safe range of WC 0.02-0.23 g g(-1) (nuts). Seeds desiccated to the safe WC and stored in LN for 2 years had similar or higher germination as seeds stored at -3A degrees C for 2 years, depending on provenance. Therefore, long-term cryopreservation of B. pendula seeds in gene banks is feasible.
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