期刊
ACTA PHYSIOLOGIAE PLANTARUM
卷 30, 期 5, 页码 729-736出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11738-008-0174-2
关键词
Suaeda salsa; A. tumefaciens; hypocotyl; GUS; transformation; halophyte
资金
- NSFC (National Natural Science Research Foundation of China [30670177, 30270793]
- Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20050445003]
Genetic manipulation technologies have been limited in the halophyte Suaeda salsa L. due to the lack of an efficient transformation system. Here, we examined factors affecting transformation and developed an efficient transformation system at the cell level using S. salsa hypocotyl as starting material. S. salsa hypocotyl explants from 10-day-old seedlings were precultured for 2 days on a hygromycin (hyg)-free callus induction medium (CIM) and then inoculated with Agrobacterium tumefaciens suspension at a concentration of 0.5 at OD600 for 5-10 min. After cocultivation with A. tumefaciens for 4 days in the dark, followed by selection on carbenicillin (carb) for 3 days, explants were placed on CIM containing 10 mg l(-1) hyg and 500 mg l(-1) carb with three to four consecutive subcultures for up to 45 days. beta-Glucuronidase assays showed an average transformation frequency of 62.89%. Gene integration was confirmed by polymerase chain reaction analysis and Northern blot analysis. To our knowledge, this is the first study to show Agrobacterium-mediated transformation in the C-3 halophyte S. salsa.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据