期刊
ACTA PHARMACOLOGICA SINICA
卷 35, 期 6, 页码 707-715出版社
ACTA PHARMACOLOGICA SINICA
DOI: 10.1038/aps.2013.197
关键词
hydrogen sulfide; homocysteine; neurotoxicity; hippocampus; apoptosis; ER stress; BDNF; tyrosine protein kinase B; Alzheimer's disease
资金
- National Natural Science Foundation of China [81202518, 81200985, 81071005]
- Natural Science Foundation of Hunan Province, China [11JJ3117, 12JJ9032]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of the People's Republic of China [[2011]508]
- Construct Program of the Key Discipline in the Hunan province
Aim: Homqcysteine (Hey) can elicit neuronal cell death, and hyperhomocysteinemia is a strong independent risk factor for Alzheimer's disease. The aim of this study was to examine the effects of hydrqgen sulfide (H2S) on Hey-induced endoplasmic reticulum (ER) stress and neuronal apoptosis in rat hippocampus. Methods: Adult male SD rats were intracerebroyentricularly (icv) injected with Hey (0.6 mu mol/d) for 7 d. Before Hey injection, the rats were treated with NaHS (30 or 100 mu mol center dot kg(-1)d(-1), ip) and/or k252a (1 mu g/d, icv) for 2 d. The apoptotic neurons were detected in hippocampal coronal slices with TUNEL staining. The expression of glucose regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), cleaved caspase-12, and BDNF in the hippocampus were examined using Western blotting assays. The generation of H2S in the hippocampus was measured with the NNDPD method. Results: Hey markedly inhibited the production of endogenous H2S and increased apoptotic neurons in the hippocampus. Furthermore, Hey induced ER stress responses in the hippocampus, as indicated by the upregulation of GRP78, CHOP, and cleaved caspase-12. Treatment with the H2S donor NaHS increased the endogenous H2S production and BDNF expression in a dose-dependent manner, and significantly reduced Hey-induced neuronal apoptosis and ER stress responses in the hippocampus. Treatment with k252a, a specific inhibitor of TrkB (the receptor of BDNF), abolished the protective effects of NaHS against Hey-induced ER stress in the hippocampus. Conclusion: H2S attenuates ER stress and neuronal apoptosis in the hippocampus of Hey-treated rats via upregulating the BDNF-TrkB pathway.
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