期刊
ACTA PHARMACOLOGICA SINICA
卷 31, 期 4, 页码 485-492出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/aps.2010.23
关键词
prostaglandin E-1; human umbilical vein endothelial cells; hydrogen peroxide; apoptosis; nitric oxide
资金
- Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University, Ji-nan, China
Aim: To investigate the protective effects of prostaglandin E-1 (PGE(1)) against H2O2-induced oxidative damage on human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were pretreated with PGE1 ( 0.25, 0.50, and 1.00 mu mol/L) for 24 h and exposed to H2O2 ( 200 mu mol/L) for 12 h, and cell viability was measured by the MTT assay. LDH, NO, SOD, GSH-Px, MDA, ROS, and apoptotic percentage were determined. eNOS expression was measured by Western blotting and real-time PCR. Results: PGE(1) (0.25-1.00 mu mol/L) was able to markedly restore the viability of HUVECs under oxidative stress, and scavenged intracellular reactive oxygen species induced by H2O2. PG(E)1 also suppressed the production of lipid peroxides, such as MDA, restored the activities of endogenous antioxidants including SOD and GSH-Px, and inhibited cell apoptosis. In addition, PGE(1) significantly increased NO content, eNOS protein, and mRNA expression. Conclusion: PGE(1) effectively protected endothelial cells against oxidative stress induced by H2O2, an activity that might depend on the up-regulation of NO expression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据