期刊
ACTA OTO-LARYNGOLOGICA
卷 134, 期 1, 页码 105-110出版社
INFORMA HEALTHCARE
DOI: 10.3109/00016489.2013.844365
关键词
Radiation treatment; tumor cell death; cell viability
Conclusion: Autophagy was involved in the radiation treatment of CNE-2 cells, and blocked autophagy enhances the radiosensitivity of nasopharyngeal carcinoma cell line CNE-2 in vitro. Objective: To determine whether autophagy induced by radiation therapy contributes to tumor cell death or represents a mechanism of resistance to therapy-mediated cell death. Methods: Autophagy in the CNE-2 nasopharyngeal carcinoma cells after radiation treatment was determined by quantitative GFP-LC3 analysis, electron microscopy, and autophagy-related molecules analysis by Western blotting. The contribution of autophagy to the cell viability was determined by MTT assay and clonogenic assay. Results: Autophagy was involved in CNE-2 cells post radiation treatment, and autophagy could ameliorate the cell viability post radiation. On the other hand, inhibition of autophagy could reduce cell viability and decrease the cell survival.
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