期刊
ACTA OTO-LARYNGOLOGICA
卷 130, 期 2, 页码 240-246出版社
TAYLOR & FRANCIS AS
DOI: 10.3109/00016480903143978
关键词
ROS; superoxide; profound hearing loss; xanthine oxidase; spectrophotometry; SDS-PAGE; cochlear implantation; stapedotomy
Conclusions: The results reported here provide the first evidence of the production of superoxide, a biologically relevant reactive oxygen species (ROS), in human inner ear perilymph (hIP) in pathological conditions, by the activity of the xanthine dehydrogenase/xanthine oxidase (XA/XO) enzyme system. Objective: To investigate the presence of ROS in hIP. Methods: Since damage and apoptosis of inner ear hair cells may occur as a result of ROS-mediated injury, we investigated the presence and production of ROS in 105 hIP samples; 98 collected from patients affected by profound sensorineural hearing loss, during surgery for cochlear implantation, and 7 controls, collected from patients affected by otosclerosis, in case of spontaneous leakage after stapedotomy. ROS production was investigated by spectrophotometric analysis and polyacrylamide gel electrophoresis (SIDS-PAGE). Results: In hIP samples tested by cytochrome c reduction kinetics, the average superoxide production was 27.34 mu moles per mg of total protein, against 0.36 in controls. Some of these hIP samples, analyzed by cytochrome c reduction kinetics in the presence of xanthine, were found to be positive for ROS-producing XA/XO enzyme. These results were supported by SDS-PAGE analysis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据