期刊
INNATE IMMUNITY
卷 21, 期 7, 页码 714-725出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/1753425915589387
关键词
Cytokine; interleukin; neutrophil; periodontitis; TNF-
资金
- Oral & Dental Research Trust [14945]
Pro-inflammatory cytokine release (IL-8, IL-6, TNF-,IL-1) by peripheral blood neutrophils, isolated from periodontitis patients (before/after therapy) and matched controls, was determined after 18h culture in the presence/absence of Escherichia coli LPS, opsonised Staphylococcus aureus, heat-killed Fusobacterium nucleatum and Porphyromonas gingivalis. All cultures demonstrated differences in the amounts of each cytokine detected (P<0.0001), with a clear release pattern (IL-8>IL-6>TNF-=IL-1). Median cytokine release from unstimulated patient neutrophils was consistently, but non-significantly, higher than from control cells. Stimulated cytokine release from untreated patient neutrophils was also consistently higher than from control cells. This hyper-reactivity was significant for all tested cytokines when data for all stimuli were combined (P<0.016). In terms of individual stimuli, significant hyper-reactivity was detected with LPS (IL-8), F. nucleatum (IL-8, TNF-), opsonised S. aureus (IL-8, TNF-, IL-1) and P. gingivalis (IL-8, IL-1). Cytokine production by patient neutrophils did not reduce following successful non-surgical periodontal therapy and, except for responses to F. nucleatum, the cytokine hyper-reactivity detected pre-therapy was retained. These data demonstrate that chronic periodontitis is characterised by neutrophils that constitutively exhibit cytokine hyper-reactivity, the effects of which could modulate local and systemic inflammatory-immune responses and influence the risk and severity of periodontitis-associated systemic inflammatory diseases.
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