期刊
INNATE IMMUNITY
卷 22, 期 2, 页码 113-123出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/1753425915619476
关键词
ROS; Streptococcus pyogenes; cellulitis; cell death; inflammation
资金
- Association de Recherche en Virologie et Dermatologie
Gram-positive Streptococcus pyogenes (group A Streptococcus or GAS) is a major skin pathogen and interacts with keratinocytes in cutaneous tissues. GAS can cause diverse suppurative and inflammatory infections, such as cellulitis, a common acute bacterial dermo-hypodermitis with a high morbidity. Bacterial isolation yields from the lesions are low despite the strong local inflammation observed, raising numerous questions about the pathogenesis of the infection. Using an invitro model of GAS-infected keratinocytes, we show that the major ROS produced is the superoxide anion (O-2(-)), and that its production is time- and dose-dependent. Using specific modulators of ROS production, we show that O-2(-) is mainly synthesized by the cytoplasmic NADPH oxidase. Superoxide anion production leads to keratinocyte necrosis but incomplete inhibition of GAS growth, suggesting that GAS may be partially resistant to the oxidative burst. In conclusion, GAS-stimulated keratinocytes are able to develop an innate immune response based on the production of ROS. This local immune response limits GAS development and induces keratinocyte cell death, resulting in the skin lesions observed in patients with cellulitis.
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