期刊
ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA
卷 58, 期 3, 页码 211-217出版社
AKADEMIAI KIADO RT
DOI: 10.1556/AMicr.58.2011.3.4
关键词
Mycobacterium tuberculosis; polymerase chain reaction; cytochrome CYP141; detection
资金
- Tehran University of Medical Sciences [9627]
Cytochrome P450 CYP141 is an intermediary metabolic and respiratory protein that interferes with oxidation reduction in Mycobacterium tuberculosis. This conserved protein has also been debated as a hypothetical target for therapeutics. We used the sequences of CYP141 gene to develop a PCR for rapid detection of Mycobacterium tuberculosis from respiratory specimens. The sensitivity of this PCR for culture positive-smear positive and culture positive-smear negative samples were 92% and 62.5%, respectively. The overall sensitivity and specificity of this PCR was 85.7% and 97.8%. As compared with other studies, it appears that the CYP141 gene is a good target for direct detection of M. tuberculosis from respiratory specimens.
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