期刊
INFECTION AND IMMUNITY
卷 83, 期 11, 页码 4266-4276出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.00653-15
关键词
-
资金
- Basic Science Research Program through the National Research Foundation of Korea - Ministry of Education [2013R1A1A2061369, 2014R1A2A2A09052791]
- Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food, and Rural Affairs, Republic of Korea [914010-04-1-HD020]
- National Research Foundation of Korea [2014R1A2A2A09052791, 2013R1A1A2061369] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
MdsABC is a Salmonella-specific tripartite efflux pump that has been implicated in the virulence of Salmonella enterica serovar Typhimurium; however, little is known about the virulence factors associated with this pump. We observed MdsABC expression-dependent alterations in the degree of resistance to extracellular oxidative stress and macrophage-mediated killing. Thinlayer chromatography and tandem mass spectrometry analyses revealed that overexpression of MdsABC led to increased secretion of 1-palmitoyl-2-stearoyl-phosphatidylserine (PSPS), affecting the ability of the bacteria to invade and survive in host cells. Overexpression of MdsABC and external addition of PSPS similarly rendered the mdsABC deletion strain resistant to diamide. Diagonal gel analysis showed that PSPS treatment reduced the diamide-mediated formation of disulfide bonds, particularly in the membrane fraction of the bacteria. Salmonella infection of macrophages induced the upregulation of MdsABC expression and led to an increase of intracellular bacterial number and host cell death, similar to the effects of MdsABC overexpression and PSPS pretreatment on the mdsABC deletion strain. Our study shows that MdsABC mediates a previously uncharacterized pathway that involves PSPS as a key factor for the survival and virulence of S. Typhimurium in phagocytic cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据