期刊
ACTA HISTOCHEMICA
卷 116, 期 1, 页码 167-175出版社
ELSEVIER GMBH
DOI: 10.1016/j.acthis.2013.07.005
关键词
Epidermal growth factor; Ischemia/reperfusion; Intestinal injury; Apoptosis; Oxidative stress; Antioxidant enzymes; Rat
类别
资金
- Scientific Research Project Coordination Unit of Istanbul University [152/20082003, UDP-1159]
Intestinal ischemia/reperfusion is a major problem which may lead to multiorgan failure and death. The aim of the Study was to evaluate the effects of epidermal growth factor (EGF) on apoptosis, cell proliferation, oxidative stress and the antioxidant system in intestinal injury induced by ischemia/reperfusion in rats and to determine if EGF can ameliorate these toxic effects. Intestinal ischemia/reperfusion injury was produced by causing complete occlusion of the superior mesenteric artery for 60 thin followed by a 60-min reperfusion period. Animals received intraperitoneal injections of 150 mu g/kg human recombinant EGF 30 min prior to the mesenteric ischemia/reperfusion. Mesenteric ischemia/reperfusion caused degeneration of the intestinal mucosa, inhibition of cell proliferation, stimulation of apoptosis and oxidative stress in the small intestine of rats. In the ischemia/reperfusion group, lipid peroxidation was stimulated accompanied by increased intestinal catalase and glutathione peroxidase activities, however, glutathione levels and superoxide dismutase activities were markedly decreased. EGF treatment to rats with ischemia/reperfusion prevented the ischemia/reperfusion-induced oxidative injury by reducing apoptosis and lipid peroxidation, and by increasing antioxidant enzyme activities. These results demonstrate that EGF has beneficial antiapoptotic and antioxidant effects on intestinal injury induced by ischemia/reperfusion in rats. (C) 2013 Elsevier GmbH. All rights reserved.
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