期刊
ACTA HISTOCHEMICA
卷 116, 期 8, 页码 1276-1288出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.acthis.2014.07.009
关键词
Nestin; Hippocampus; Trimethyltin; Dentate gyrus; Astrocytes; Mice
类别
资金
- Animal Medical Institute of Chonnam National University
- National Research Foundation of Korea - Ministry of Science, ICT and Future Planning [NRF-2012R1A1B4001262]
- Next-Generation BioGreen 21 Program - Rural Development Administration, Republic of Korea [PJ009617]
Nestin is a protein of embryonic intermediate filaments expressed by multipotent neural stem cells. In the present study, the nestin expression pattern in the mouse hippocampus 1, 2, 3, 4, and 8 days after treatment with trimethyltin (TMT) was examined to explore the possible role played by nestin in chemically induced hippocampal injury. TMT treatment (2.5 mg/kg, intraperitoneally) selectively injured the dentate gyrus (DG) of the mouse hippocampus. The level of hippocampal mRNA encoding nestin increased significantly 2 and 3 days post-treatment and thereafter decreased (at 4 and 8 days post-treatment). The level of nestin protein significantly increased 2 - 4 days post-treatment, particularly in the injured region of the DG, and predominantly in glial fibrillary acidic protein-positive astrocytes in the hippocampal DG. Ki67-positive proliferating cells were increased following TMT treatment and colocalized with nestin-positive reactive astrocytes. Thus, we suggest that nestin contributes to remodeling of the chemically injured DG via glial scar formation and the alteration of neurogenesis. (C) 2014 Elsevier GmbH. All rights reserved.
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