期刊
ACTA HISTOCHEMICA
卷 113, 期 5, 页码 564-569出版社
ELSEVIER GMBH
DOI: 10.1016/j.acthis.2010.06.003
关键词
Hedgehog pathway; Lung cancer; Smoothened; Rab23; PDGFR alpha; Hedgehog interacting protein
类别
资金
- National Science Foundation of China [30228031, 30671072, 30570967]
- Ministry of Science and Technology of China [2007CB947100, 2007CB815800]
- NIH [R01-CA94160]
- Department of Defense [DOD-PC030429]
- NIEHS Center at UTMB
Abnormal hyperactivation of the hedgehog (Hh) pathway has been reported in many types of human cancers, including lung cancer. However, most reports are based on studies of fewer than three Hh target genes and the data vary between different studies. In the present report, we have determined the expression levels of several important components of the Hh pathway in lung cancers by using RT-PCR, in situ hybridization and immunohistochemistry. These molecules include Smoothened (SMO), Rab23, the downstream target platelet-derived growth factor receptor alpha (PDGFR alpha), hedgehog interacting protein (HIP) and hepatocyte nuclear factor 3-beta (HNF3 beta). Our data show that some components of the hedgehog pathway, such as SMO, Rab23 and PDGFR alpha are expressed in many lung cancer specimens, although other hedgehog target genes are infrequently detected in lung cancer. Loss of HIP expression was found in several cases of lung cancers. Our study indicates that there might be some additional mechanisms involved in the hyperactivation of the Hh pathway. Thus, we suggest that lung cancer with heterogeneous tumor type harboring Hh signaling activation may have some novel and different regulatory mechanisms. (C) 2010 Elsevier GmbH. All rights reserved.
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