期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 66, 期 -, 页码 246-254出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2015.01.002
关键词
Maceration; Ultrasound-assisted extraction; Folin-Ciocalteu; Bioactive compounds; HPLC-ESI-QTOF-MS
资金
- Spanish Ministry of Science and Innovation [AP2010-1551, PTA2012-6956-E]
- Andalusian Regional Government Council of Innovation and Science [P11-CTS-7625]
Moringa oleifera Lam is considered one of the most useful tree in the world because every part of the Moringa tree can be used such as nutritional supplement, for medication, and industrial purposes. Conventional solid-liquid extraction and ultrasound-assisted extraction (UAE) were performed using different solvents and mixtures of solvents with water. The total phenolic content was determined using Folin-Ciocalteu assay. UAE using ethanol :water (50:50) was the best extraction procedure, which allowed 47 +/- 4 mg gallic acid equivalents (GAE)/g dry leaf to be obtained. In addition, high-performance liquid chromatography coupled to electrospray ionization quadropole-time of flight mass spectrometry (HPLC-ESI-QTOF-MS) was used to characterize the bioactive compounds in the resulting extract. Consequently, 59 compounds were tentatively characterized, phenolic acid derivatives and flavonoids being the most abundant. Furthermore, 30 of these compounds were tentatively identified for the first time in M. oleifera leaves. This study shows that leaves from M. oleifera are a good nutritional resource used as a nutritional supplement and may carry additional opportunities for food ingredient innovations, pharmaceutical and cosmetics products. (C) 2015 Elsevier B.V. All rights reserved.
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