Ultrasound extraction of phenolics and anthocyanins from jabuticaba peel

Jabuticaba is a dark berry rich in phytochemicals such as polyphenols and anthocyanins. In the present study, ultrasound assisted extraction (UAE) of bioactive compounds from jabuticaba peels was evaluated. Ethanol was used as solvent because it is renewable (obtained from sugar cane) and GRAS. The effect of solvent concentration, pH and the extraction time on the target compounds (anthocyanins and phenolics) was evaluated by response surface methodology. The solvent to ratio was 1:20 and the extraction temperature was 30 degrees C. The anthocyanins and phenolics extracted were analyzed by LC-MS and HPLC and identified as cyanidin-3-0-glucoside (anthocyanin) and ellagic acid (phenol). The operating condition that has maximized the target compounds extraction required the sonicated of the peels for 10 min in a 46% (v/v) ethanol:water solution acidified at pH 1. The extraction yielded 4.8 mg/g dry peel of monomeric anthocyanin; 92.8 mg/g dry peel of gallic acid equivalent; 4.9 mg/g dry peel of cyanidin-3-O-glucoside and 7.8 mg/g dry peel of ellagic acid. The results showed that with an adequate operating condition it was possible to reach good yields using a simple extraction process. (C) 2015 Elsevier B.V. All rights reserved.