期刊
ACTA DIABETOLOGICA
卷 50, 期 5, 页码 753-761出版社
SPRINGER-VERLAG ITALIA SRL
DOI: 10.1007/s00592-012-0410-5
关键词
Gut microbiota; Obesity; Metabolic diseases; Insulin action; DGGE
资金
- Ministerio de Educacion y Ciencia [SAF2008-0273, MTM2008-0064]
- Agence Nationale de la Recherche (ANR FLORADIP)
- Agence Nationale de la Recherche (FLORINFLAM)
- Societe Franco-phone du Diabete-Glaxo Smith Kline (SFD-GSK)
- Benjamin Delessert foundation
The role of the gut microbiota in the induction of metabolic diseases has now been increasingly recognized worldwide. Indeed, a specific gut microbiota has been shown to characterize lean versus obese phenotypes both in humans and mice. We have also recently demonstrated that a precise gut microbiota is associated with the host's responsiveness to a high-fat diet. Therefore, we hypothesized that insulin resistance in humans could also be linked to a specific gut microbiota. To this aim, microbial DNA and RNA were extracted from the appendix contents of insulin-resistant versus insulin-sensitive obese subjects, matched for body mass index and age, and analyzed by DNA- and RNA-DGGE. Microbial DNA analysis showed that the patients fully segregated according to their degree of insulin action. Conversely, microbial RNA investigation showed that some degree of homology still existed between insulin-sensitive and insulin-resistant patients. Quantitative trait analysis, ordinary least squares regression, principal components regression, partial least squares, canonical correlation analysis, and canonical correspondence analysis also showed a net separation of the two phenotypes analyzed. We conclude that a specific gut microbial profile is associated with insulin action in humans.
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