Preparation of Anti-idiotype Antibodies of O, O-Dimethyl Organophosphorus Pesticides by Phage Display Technology

Anti-idiotype antibody (AId or Ab2) can be applied to develop noncompetitive immunoassay for hapten molecules. In this study, hapten molecules were immobilized on sepharose, and then used for antibody purification. Affinity purified positive and negative primary antibodies were used for bio-panning of anti-idiotype antibody from phage display antibody library. After three round of panning, 3 alpha-AId and 9 beta-AId clones were achieved. Clones D11 and B9, identified as beta-AId and alpha-AId respectively, were selected for developing a noncompetitive immunoassay system. The IC50 value and detection limit of this method for malathion were calculated to be (113. 7 +/- 34. 2) mu g/L and 10. 5 mu g/L, respectively.