期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 72, 期 -, 页码 142-151出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2014.12.047
关键词
Lysozyme stability; Edible films; Extrusion; Sodium caseinate; Active packaging
资金
- Region Rhone-Alpes
- Fonds pour le Developpement de la Recherche du Technopole Alimentec
Sodium caseinate-based edible/biodegradable films containing lysozyme were prepared directly by extrusion processes to obtain antimicrobial films. Thermoplastic pellets containing I% (w/w) lysozyme were blown by an industrial-like blown-film-extruder to obtain thermoplastic thin films. Lysozyme stability depended mainly on processing temperature and glycerol content. A correct choice of these parameters allowed obtaining pellets and films with 40.8 +/- 0.5% and 26.4 +/- 0.6% lysozyme residual activity, respectively. Whatever the storage temperature (-20 degrees C or room temperature), lysozyme activity of pellets remained constant for at least 5 weeks of storage. Antimicrobial activity against Micrococcus luteus was assayed in Tryptone Soya Broth (TSB): no bacterial growth was observed for 25 h at 37 degrees C in the presence of 0.05 g.L-1 pellets, while a 2.4 log increase of bacterial population was observed in the presence of control pellets without lysozyme. Traditional properties of films such as their water sorption and mechanical properties were also investigated and proved that addition of lysozyme at a 1% concentration did not affect these properties as well as their ultimate aerobic biodegradability. The application potential of such edible antimicrobial films is discussed in relation with these properties. (C) 2014 Elsevier B.V. All rights reserved.
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