Genotype and environment effects on tocopherol and plastochromanol-8 contents of winter oilseed rape doubled haploid lines derived from F1 plants of the cross between yellow and black seeds

To determine the effect of genotype and the environment on tocopherol (T) and plastochromanol-8 (PC-8) contents in winter oilseed rape (Brassica napus L.), a three-year study was performed. The plant material consisted of 25 doubled haploid (DH) lines derived from the F-1 hybrid obtained from the cross between two DH lines (Z x H), (Z-114-yellow seeds and H-2-26-black seeds). Quantitative contents of alpha-T, beta-T, gamma-T, delta-T and PC-8 were determined by HPLC. Total tocopherol content ranged between 40.62 and 51.20 mg/100 g and PC-8 5.43-8.92 mg/100 g. The alpha-T/gamma-T ratio ranged from 0.77 to 1.23. The heritability in the broad sense was examined for the following components: PC-8, alpha-T, beta-T, gamma-T, delta-T, alpha/gamma-T and gamma-T contents in oil. Heritability levels were 0.60, 0.58, 0.53, 0.50, and 0.43, respectively. This suggest the superiority of the effect of genotype on tocopherol contents. The h(2) values for delta-T and beta-T were 0.36 and 0.32, which demonstrates a great influence of environmental conditions. Among all analyzed genotypes, eleven were selected as satisfactory and stable regarding tocopherol or PC-8 contents. (C) 2014 Elsevier B.V. All rights reserved.