期刊
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
卷 69, 期 -, 页码 460-462出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1744309113006490
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资金
- National Basic Research Program of China (973 Program) [2011CB100700, 2010 CB911800]
- National S&T Major Project on Major Infectious Diseases from the Ministry of Science and Technology of the People's Republic of China [2012ZX10001-008]
The effector protein MoHrip1 from the pathogenic fungus Magnaporthe oryzae was purified and crystallized using the sitting-drop vapour-diffusion method. Native crystals appeared in a solution composed of 0.005 M cobalt(II) chloride hexahydrate, 0.005 M nickel(II) chloride hexahydrate, 0.005 M cadmium chloride hydrate, 0.005 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 12%(w/v) polyethylene glycol 3350. A native data set was collected to 1.9 angstrom resolution at 100 K using an in-house X-ray source. The structure of MoHrip1 was successfully determined by molecular replacement using a homologous structure.
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