Production, purification, crystallization and structure determination of H-1 Parvovirus

Crystals of H-1 Parvovirus (H-1PV), an antitumor gene-delivery vector, were obtained for DNA-containing capsids and diffracted X-rays to 2.7 angstrom resolution using synchrotron radiation. The crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 255.4, b = 350.4, c = 271.6 angstrom, beta = 90.34 degrees. The unit cell contained two capsids, with one capsid per crystallographic asymmetric unit. The H-1PV structure has been determined by molecular replacement and is currently being refined.