期刊
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
卷 68, 期 -, 页码 802-805出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1744309112020556
关键词
-
资金
- National Basic Research Program of China (973 Program) [2010CB911800, 2011CB100700]
- Ministry of Science and Technology of the People's Republic of China [2012ZX10001-008]
The effector protein PevD1 from the pathogenic fungus Verticillium dahliae was purified and crystallized using the hanging-drop vapour-diffusion method. Native crystals appeared in a solution consisting of 4.0 M sodium formate. A native data set was collected at 1.9 angstrom resolution at 100 Kusing an in-house X-ray source. Because of the absence of useful methinione in the protein sequence, derivative crystals that contained iodine were obtained by soaking in 1.25 M potassium iodide, and a data set that contained anomalous signal was collected using the same X-ray facility at a wavelength of 1.54 angstrom. The single-wavelength anomalous dispersion method was used to successfully solve the structure based on the anomalous signal generated from iodine.
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