期刊
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
卷 66, 期 -, 页码 1552-1556出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1744309110039801
关键词
CRISPR-associated proteins; Cas2; Desulfovibrio vulgaris
资金
- NIH [GM63611]
CRISPRs (clustered regularly interspaced short palindromic repeats) provide bacteria and archaea with RNA-guided acquired immunity to invasive DNAs. CRISPR-associated (Cas) proteins carry out the immune effector functions. Cas2 is a universal component of the CRISPR system. Here, a 1.35 A resolution crystal structure of Cas2 from the bacterium Desulfovibrio vulgaris (DvuCas2) is reported. DvuCas2 is a homodimer, with each protomer consisting of an N-terminal beta alpha beta beta alpha beta ferredoxin fold (amino acids 1-78) to which is appended a C-terminal segment (amino acids 79-102) that includes a short 3(10)-helix and a fifth beta-strand. The beta 5 strands align with the beta 4 strands of the opposite protomers, resulting in two five-stranded antiparallel beta-sheets that form a sandwich at the dimer interface. The DvuCas2 dimer is stabilized by a distinctive network of hydrophilic cross-protomer side-chain interactions.
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