Application of Biosensor Surface Immobilization Methods for Aptamers

Aptamer with small molecular weight, simple structure and easy synthesis,. repeatable usage and long-term preservation, has important applications in biosensor field. Several common used methods for aptamer fixed onto the sensor surface were introduced in this view, including the gold-sulfur self-assembled monolayers, covalent bond, biotin/avidin affinity, complementary nucleic acid chain connection, as well as current research progress and the characteristics of each method. The wide detection range, low detection limit, short detection time and multi-detection will be the development trend and emphasis for aptasensors used in food quality and safety testing, disease diagnosis, drug analysis, environment monitoring, etc.