期刊
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
卷 52, 期 3, 页码 1243-1251出版社
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.10-5962
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资金
- MRC [G0600755, G0501050]
- Wellcome Trust [074165]
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Royal Society
- MRC [G0600755, G0501050] Funding Source: UKRI
- Medical Research Council [G0501050, G0600755] Funding Source: researchfish
PURPOSE. Retinopathy, globe enlarged (RGE) is an inherited genetic disease of chickens with a corneal phenotype characterized by loss of tissue curvature and changes in peripheral collagen fibril alignment. This study aimed to characterize the material behavior of normal and RGE chicken corneas wider inflation and compare this with new spatial- and depth-resolved microstructural information to investigate how stromal fibril architecture determines corneal behavior under intraocular pressure (IOP). METHODS. Six RGE chicken corneas and six age-matched normal controls were tested using trephinate inflation and their stress-strain behavior determined as a function of posterior pressure. Second harmonic generation mulitphoton microscopy was used to compare the in-plane appearance and degree of through-plane interlacing of collagen lamellae between normal and mutant corneas. RESULTS. RGE corneas displayed a 30-130% increase in material stiffness [E-tangent(RGE) = 0.94 +/- 0.18 MPa to 3.09 +/- 0.66 MPa; E-tangent(normals) = 0.72 +/- 0.13 MPa to 1.34 +/- 0.35 MPa] (P <= 0.05). The normal in-plane disposition of anterior collagen in the peripheral cornea was altered in RGE but through-plane lamellar interlacing was unaffected. CONCLUSIONS. This article demonstrates changes in corneal material behavior in RGE that are qualitatively consistent with microstructural collagen alterations identified both herein and previously. This study indicates that, in general, changes in stromal fibril orientation may significantly affect conical material behavior and thereby its response to IOP. (Invest Ophthalmol Vis Sci. 2011;52:1243-1251) DOI:10.1167/iovs.10-5962
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