期刊
INDIAN JOURNAL OF PHARMACOLOGY
卷 47, 期 3, 页码 292-298出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/0253-7613.157127
关键词
Carrageenan; ellagic acid; inducible nitric oxide synthase; prostaglandin E-2; Rat
资金
- Ahvaz Jundishapur University of Medical Sciences [PRC150]
Objectives: Ellagic acid (EA) has shown antinociceptive and anti-inflammatory effects. Inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2) enzymes and also cytokines play a key role in many inflammatory conditions. This study was aimed to investigate the mechanisms involved in the anti-inflammatory effect of EA. Materials and Methods: Carrageenan-induced mouse paw edema model was used for induction of inflammation. Results: The results showed that intraplantar injection of carrageenan led to time-dependent development of peripheral inflammation, which resulted in a significant increase in the levels of tumor necrosis factor alpha (TNF-alpha) and interleukin 1 (IL-1) beta, nitric oxide (NO) and prostaglandin E-2 (PGE(2)) and also iNOS and COX-2 protein expression in inflamed paw. However, systemic administration of EA (1-30 mg/kg, intraperitoneal [i.p.]) could reduce edema in a dose-dependent fashion in inflamed rat paws with ED50 value 8.41 (5.26-14.76) mg/kg. It decreased the serum concentration of NO, PGE(2), aspartate aminotransferase and alanine aminotransferase, and suppress the protein expression of iNOS, COX-2 enzymes, and attenuated the formation of PGE(2), TNF-alpha and IL-1 beta in inflamed paw tissue. We also demonstrated that EA significantly decreased the malondialdehyde (MDA) level in liver at 5 h after carrageenan injection. Moreover, histopathological studies indicated that EA significantly diminished migration of polymorphonuclear leukocytes into site of inflammation, as did indomethacin. Conclusions: Collectively, the anti-inflammatory mechanisms of EA might be related to the decrease in the level of MDA, iNOS, and COX-2 in the edema paw via the suppression of pro-inflammatory cytokines (TNF alpha, IL1 beta), NO and PGE(2) overproduction.
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