Callus induction and proliferation from Rosa hybrida leaf explants

To establish a resourceful tissue culture system to induce anthocyanin pigments, research was conducted to obtain high quality callus from leaf explant of Rosa hybrida cv. Pusa Ajay. Of the different treatments employed for callus induction using Murashige and Skoog (MS) medium, a treatment combination of 19.60 mu M indole-3-butyric acid (IBA) + 4.65 mu M kinetin (Kin) + 108.6 mM adenine sulphate (AdS) gave the maximum induction coefficient (97.75 +/- 1.11%) along with maximum gain in callus biomass accumulation. This treatment also resulted in the lowest fresh- and dry- cell weight ratio (26.52) suggesting actual gain in callus biomass accumulation. Correlation analysis indicated a significant positive correlation between callus induction coefficient and cell fresh- (r = 0.939) and dry- (r = 0.951) weight.